A novel bacterial water Hypersensitivity-like protein shows
Details of Research
TitleA novel bacterial water Hypersensitivity-like protein shows in vivo protection against cold and freeze damageAbstractMetagenomic library screening, by functional or sequence analysis, has become an established method for the identification of novel genes and gene products, including genetic elements implicated in microbial stress response and adaptation. We have identified, using a sequence-based approach, a fosmid clone from an Antarctic desert soil metagenome library containing a novel gene which codes for a protein homologous to a Water Hypersensitivity domain (WHy). The WHy domain is typically found as a component of specific LEA (Late Embryogenesis Abundant) proteins, particularly the LEA-14 (LEA-8) variants, which occur widely in plants, nematodes, bacteria and archaea and which are typically induced by exposure to stress conditions. The novel WHy-like protein (165 amino acid, 18.6 kDa) exhibits a largely invariant NPN motif at the N-terminus and has high sequence identity to genes identified in Pseudomonas genomes. Expression of this protein in Escherichia coli significantly protected the recombinant host against cold and freeze stress. Copyright FEMS 2015.AcknowledgementsThe authors gratefully acknowledge support from the South African National Antarctic Program of the National Research Foundation. DAC and DEA wish to thank Professor Roberta Farrell (University of Waikato, New Zealand) for providing access to the Antarctic McMurdo Dry Valleys through the Antarctica-NZ 2011 K021 field expedition.
1st AuthorAnderson, D.AuthorAnderson, D.Ferreras, E.Trindade, M.Cowan, D.Year2015JournalFEMS Microbiology LettersVolume362Number15DOI10.1093/femsle/fnv110URLhttps://www.scopus.com/inward/recor.....c5562f7322beb111be7e4e26cKeywordsamino acidbacterial proteinbacterial water Hypersensitivity like proteinLEA 14 proteinLEA 8 proteinLEA proteinunclassified drugbacterial proteinsoilwater, adaptationamino terminal sequencearchaeonArticlebacterial geneticsbacteriumcold stresscontrolled studyEscherichia colifosmidfreezinggene productgenetic codegenetic identificationhomologous recombinationin vivo studymetagenomicsnematodenonhumannucleotide sequenceplantpriority journalprotein domainprotein expressionprotein motifPseudomonassequence analysisstressamino acid sequenceAntarcticabacterial genebiologydesiccationDNA sequencegene librarygeneticsmetagenomemicrobiologyphylogenyphysiological stressphysiologysoil, Adaptation, PhysiologicalAmino Acid SequenceAntarctic RegionsBacterial ProteinsComputational BiologyDesiccationEscherichia coliFreezingGene LibraryGenes, BacterialMetagenomePhylogenyPseudomonasSequence Analysis, DNASoilSoil MicrobiologyStress, PhysiologicalWater, rank5Author KeywordsAntarctic soilDesiccation toleranceFreeze-thawFunctional metagenomicsLate embryogenesis abundant proteinWater Hypersensitivity protein
TypeArticleCitationAnderson, D., Ferreras, E., Trindade, M. and Cowan, D. (2015). A novel bacterial water Hypersensitivity-like protein shows in vivo protection against cold and freeze damage. FEMS Microbiology Letters, 362(15)
Antarctica NZ (29th Nov 2018). A novel bacterial water Hypersensitivity-like protein shows . In Website Antarctica NZ. Retrieved 26th Jan 2021 07:11, from https://adam.antarcticanz.govt.nz/nodes/view/63364