Characterisation of bacterioplankton communities in the melt

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TitleCharacterisation of bacterioplankton communities in the meltwater ponds of Bratina Island, Victoria Land, AntarcticaAbstractA unique collection of Antarctic aquatic environments (meltwater ponds) lies in close proximity on the rock and sediment-covered undulating surface of the McMurdo Ice Shelf, near Bratina Island (Victoria Land, Antarctica). During the 2009-10 mid-austral summer, sets of discrete water samples were collected across the vertical geochemical gradients of five meltwater ponds (Egg, P70E, Legin, Salt and Orange) for geochemical and microbial community structure analysis. Bacterial DNA fingerprints (using Automated Ribosomal Intergenic Spacer Analysis) statistically clustered communities within ponds based on anosim (R = 0.766, P = 0.001); however, one highly stratified pond (Egg) had two distinct depth-related bacterial communities (R = 0.975, P = 0.008). 454 pyrosequencing at three depths within Egg also identified phylum level shifts and increased diversity with depth, Bacteroidetes being the dominant phyla in the surface sample and Proteobacteria being dominant in the bottom two depths. best analysis, which attempts to link community structure and the geochemistry of a pond, identified conductivity and pH individually, and to a lesser extent Ag109, NO2 and V51 as dominant influences to the microbial community structure in these ponds. Increasing abundances of major halo-tolerant OTUs across the strong conductivity gradient reinforce it as the primary driver of community structure in this study. Copyright 2014 Federation of European Microbiological Societies.AcknowledgementsThis Research was funded by New Zealand Post and logistical support was provided by Antarctica New Zealand through their postgraduate research programme (project number K160). Support was also provided by the International Centre for Terrestrial Antarctic Research (ICTAR) at the University of Waikato. We are grateful to Sarah Kelly for assistance with 454 sample preparation and Charles Lee for sequence data processing and other advice during the project.

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1st AuthorArcher, S.AuthorArcher, S.McDonald, I.Herbold, C.Cary, S.Year2014JournalFEMS Microbiology EcologyVolume89Number2Pages451-464DOI10.1111/1574-6941.12358URLhttps://www.scopus.com/inward/recor.....a17bca832bfe2e29e1fde8486Keywordsrank5

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TypeArticleCitationArcher, S., McDonald, I., Herbold, C. and Cary, S. (2014). Characterisation of bacterioplankton communities in the meltwater ponds of Bratina Island, Victoria Land, Antarctica. FEMS Microbiology Ecology, 89(2): 451-464 IdentifierArcher2014Relevancerank5

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Cary, S.