Changes in physiological responses of an Antarctic fish, the
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TitleChanges in physiological responses of an Antarctic fish, the emerald rock cod (Trematomus bernacchii), following exposure to polybrominated diphenyl ethers (PBDEs)
AbstractAlthough polybrominated diphenyl ethers (PBDEs) have the ability to undergo long-range atmospheric transport to remote ecosystems like Antarctica, a recent study found evidence for a local source within the Antarctic. PBDEs from sewage treatment outfalls of McMurdo Station and Scott Base on Ross Island have been attributed to the high concentrations measured in emerald rock cod (Trematomus bernacchii). The potential impact of PBDEs on Antarctic fish physiology is unknown and therefore, the aim of this study was to obtain a greater understanding of physiological responses of emerald rock cod for assessing changes in ecosystem quality. A PBDE mixture (ΣPBDE 8 congeners) was administered fortnightly over 42 days and physiological changes were observed throughout this period and for a further 14 days thereafter. Changes in liver composition, molecular level changes and enzyme activities of selected detoxification-mediated and antioxidant defence markers were measured. Changes in total lipid, lipid peroxide and protein carbonyl concentrations in emerald rock cod liver were consistent with increases in nucleus surface area in the PBDE-treated groups, suggesting alterations in cellular function. Changes in the activities of selected antioxidant enzymes indirectly indicated oxidative stress, possibly resulting in the changes in liver composition. Additionally, glutathione-S-transferase (GST) activity reached its peak faster than that of ethoxyresorufin-O-deethylase (EROD), suggesting that during the early response to PBDE exposures there could be a greater involvement of GST-mediated detoxification. Thus, for at least the species examined here, protein carbonyl and lipid peroxides were useful and informative biomarkers for cellular level responses following PBDE-related exposure. Furthermore, our findings suggest that emerald rock cod exposed to PBDEs develop oxidative stress - a condition with potential consequences for fish growth, health and reproduction. Copyright 2012 Elsevier B.V.AcknowledgementsThis work was supported by the Todd Foundation for Excellence (Wellington, New Zealand) and Antarctica New Zealand (Christchurch, New Zealand) [K068]. We would like to acknowledge Pfizer (Australia) for donating the gelatine capsules; Simon Morley, Todd Beaumont, Amanda Bates and Jessica Ericson for assistance with capturing fish; Beverly Dickson (Portobello Marine Laboratory, University of Otago), Gavin Robinson and Jan McKenzie (School of Biological Sciences, University of Canterbury) for assistance with the dose study; Yuichi Ozaki and Alvin Setiawan (Department of Zoology, University of Otago) for assistance with molecular techniques; and Ken Miller (Department of Zoology, University of Otago) for assistance with the design of Fig. 1.
AbstractAlthough polybrominated diphenyl ethers (PBDEs) have the ability to undergo long-range atmospheric transport to remote ecosystems like Antarctica, a recent study found evidence for a local source within the Antarctic. PBDEs from sewage treatment outfalls of McMurdo Station and Scott Base on Ross Island have been attributed to the high concentrations measured in emerald rock cod (Trematomus bernacchii). The potential impact of PBDEs on Antarctic fish physiology is unknown and therefore, the aim of this study was to obtain a greater understanding of physiological responses of emerald rock cod for assessing changes in ecosystem quality. A PBDE mixture (ΣPBDE 8 congeners) was administered fortnightly over 42 days and physiological changes were observed throughout this period and for a further 14 days thereafter. Changes in liver composition, molecular level changes and enzyme activities of selected detoxification-mediated and antioxidant defence markers were measured. Changes in total lipid, lipid peroxide and protein carbonyl concentrations in emerald rock cod liver were consistent with increases in nucleus surface area in the PBDE-treated groups, suggesting alterations in cellular function. Changes in the activities of selected antioxidant enzymes indirectly indicated oxidative stress, possibly resulting in the changes in liver composition. Additionally, glutathione-S-transferase (GST) activity reached its peak faster than that of ethoxyresorufin-O-deethylase (EROD), suggesting that during the early response to PBDE exposures there could be a greater involvement of GST-mediated detoxification. Thus, for at least the species examined here, protein carbonyl and lipid peroxides were useful and informative biomarkers for cellular level responses following PBDE-related exposure. Furthermore, our findings suggest that emerald rock cod exposed to PBDEs develop oxidative stress - a condition with potential consequences for fish growth, health and reproduction. Copyright 2012 Elsevier B.V.AcknowledgementsThis work was supported by the Todd Foundation for Excellence (Wellington, New Zealand) and Antarctica New Zealand (Christchurch, New Zealand) [K068]. We would like to acknowledge Pfizer (Australia) for donating the gelatine capsules; Simon Morley, Todd Beaumont, Amanda Bates and Jessica Ericson for assistance with capturing fish; Beverly Dickson (Portobello Marine Laboratory, University of Otago), Gavin Robinson and Jan McKenzie (School of Biological Sciences, University of Canterbury) for assistance with the dose study; Yuichi Ozaki and Alvin Setiawan (Department of Zoology, University of Otago) for assistance with molecular techniques; and Ken Miller (Department of Zoology, University of Otago) for assistance with the design of Fig. 1.Details
1st AuthorGhosh, R.AuthorGhosh, R.Lokman, P.Lamare, M.Metcalf, V.Burritt, D.Davison, W.Hageman, K.Year2013JournalAquatic ToxicologyVolume128-129Pages91-100DOI10.1016/j.aquatox.2012.11.019URLhttps://www.scopus.com/inward/recor.....85cdbdf74338af99e1aaf58c2Keywordsbiological markercarbonyl derivativecatalasecopper zinc superoxide dismutaseethoxyresorufin deethylaseglutathione peroxidaseglutathione transferaselipidlipid peroxidemessenger RNApolybrominated diphenyl ether, antioxidantbiomanipulationdetoxificationecosystem resilienceenzyme activityfishgrowth responselong range transportPBDEphysiological responsepoint source pollutionpollution effectpollution exposure, animal cellanimal experimentanimal modelanimal tissueantioxidant activityarticlebiochemical compositioncell functioncellular stress responsecontrolled studyCYP1A1 genedetoxificationecotoxicityenvironmental exposureenzyme activityfemalefishgenelipid peroxidationliver toxicitylong term exposuremarine environmentnonhumanoxidative stresspriority journalquantitative analysisSOD geneTrematomus bernacchiiwater pollution, AnimalsAntarctic RegionsBiological MarkersEnvironmental ExposureFish ProteinsGene Expression ProfilingGene Expression RegulationHalogenated Diphenyl EthersLiverPerciformesWater Pollutants, Chemical, Antarctica, Lotella rhacinaSebastes schlegeliTrematomus bernacchii, rank5Author KeywordsAntarctic fishChronic exposureNotothenioidPollution and research basesPolybrominated diphenyl ethers
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TypeArticleCitationGhosh, R., Lokman, P., Lamare, M., Metcalf, V., Burritt, D., Davison, W. and Hageman, K. (2013). Changes in physiological responses of an {Antarctic} fish, the emerald rock cod (Trematomus bernacchii), following exposure to polybrominated diphenyl ethers (PBDEs). Aquatic Toxicology, 128-129: 91-100 IdentifierGhosh2013Relevancerank5
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Hageman, K., Changes in physiological responses of an Antarctic fish, the , [Ghosh2013]. Antarctica NZ, accessed 14/05/2025, https://adam.antarcticanz.govt.nz/nodes/view/63510, 10.1016/j.aquatox.2012.11.019