Trapping and imaging of micron-sized embryos using dielectro

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TitleTrapping and imaging of micron-sized embryos using dielectrophoresisAbstractDevelopment of dielectrophoretic (DEP) arrays for real-time imaging of embryonic organisms is described. Microelectrode arrays were used for trapping both embryonated eggs and larval stages of Antarctic nematode Panagrolaimus davidi. Ellipsoid single-shell model was also applied to study the interactions between DEP fields and developing multicellular organisms. This work provides proof-of-concept application of chip-based technologies for the analysis of individual embryos trapped under DEP force. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.AcknowledgementsGrant sponsor: Supported by the Endeavour Research Fellowships, Department of Education, Employment and Workplace Relations, Australia and the Centre for Intelligent Systems Research, Deakin University, Australia (K. K.); Foundation for Research Science and Technology (FRST), New Zealand (D. W., D. E. W.); Faculty Research and Development Fund, University of Auckland. New Zealand (D. W.); and BBSRC, EPSRC and Scottish Funding Council, United Kingdom, funded under the RASOR Program (J. M. C.).

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1st AuthorKhoshmanesh, K.AuthorKhoshmanesh, K.Kiss, N.Nahavandi, S.Evans, C.Cooper, J.Williams, D.Wlodkowic, D.Year2011JournalElectrophoresisVolume32Number22Pages3129-3132DOI10.1002/elps.201100160URLhttps://www.scopus.com/inward/recor.....21ea412b19aadf47c11c1bfa1Keywordsarticleelectric fieldelectrophoresisellipsometryembryofeasibility studyimage analysislab on a chiplarvalocomotionmicroarray analysismicroelectrodemicrofluidicsnematodenonhumanPanagrolaimus davidi, AnimalsElectrophoresisLarvaMicrofluidic Analytical TechniquesRhabditida, rank3Author KeywordsDielecrophoresisEmbryoLab-on-a-chipMicrofluidicsReal-time imaging

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TypeArticleCitationKhoshmanesh, K., Kiss, N., Nahavandi, S., Evans, C., Cooper, J., Williams, D. and Wlodkowic, D. (2011) .Trapping and imaging of micron-sized embryos using dielectrophoresis. Electrophoresis, 32(22): 3129-3132 IdentifierKhoshmanesh2011Relevancerank3