Aerobic anoxygenic phototrophic bacteria in Antarctic sea ic

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TitleAerobic anoxygenic phototrophic bacteria in Antarctic sea ice and seawaterAbstractAerobic anoxygenic phototrophs are obligate aerobes with unusually high concentrations of carotenoids, low cellular contents of bacteriochlorophyll-a and they lack light-harvesting complex II. In this study, sea ice and seawater samples were collected from six different sites in the Ross Sea, Antarctica. Using a combination of primers for pufM (which encodes a pigment-binding protein subunit of the reaction centre complex), clone libraries of DNA and cDNA were created and a total of 63 positive clones were obtained from three sites, all clustering within the Î±-Proteobacteria. Fifty-three of these clones were from seawater. The remaining clones were from sea ice and all were found in the middle and bottom sections of the ice. These sea ice bacteria may favour the lower part of the ice matrix where irradiance is low. This report highlights the first findings of AAnPs in antarctic sea ice and seawater within the Ross Sea Region. © 2011 Society for Applied Microbiology and Blackwell Publishing Ltd.AcknowledgementsThe authors thank Event K043 for their help with field sampling during the field seasons of 2007 and 2008, and acknowledge the logistical and field support of Antarctica New Zealand, in particular S. Gordon, Project Manager of the Latitudinal Gradient Project (LGP). We thank Bex Cowie for the preparation of the cDNAs. We also thank both anonymous reviewers for their suggestions to improve the manuscript. This research was supported by the New Zealand Foundation of Research Science and Technology Grant number VICX0706 to KGR, the VUW SFRG Grants 103167/2585 and 96429/2661 to EYK and WP respectively. The authors declare no conflict in interests.

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1st AuthorKoh, E.AuthorKoh, E.Phua, W.Ryan, K.Year2011JournalEnvironmental Microbiology ReportsVolume3Number6Pages710-716DOI10.1111/j.1758-2229.2011.00286.xURLhttps://www.scopus.com/inward/recor.....148ac8e91d059ec9f626ad23fKeywordsAlphaproteobacteriaBacteria (microorganisms)Photobacteria, rank5

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TypeArticleCitationKoh, E., Phua, W. and Ryan, K. (2011). Aerobic anoxygenic phototrophic bacteria in Antarctic sea ice and seawater. Environmental Microbiology Reports, 3(6): 710-716