Influence of soil properties on archaeal diversity and distr

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TitleInfluence of soil properties on archaeal diversity and distribution in the McMurdo Dry Valleys, AntarcticaAbstractArchaea are the least understood members of the microbial community in Antarctic mineral soils. Although their occurrence in Antarctic coastal soils has been previously documented, little is known about their distribution in soils across the McMurdo Dry Valleys, Victoria Land. In this study, terminal-restriction fragment length polymorphism (t-RFLP) analysis and 454 pyrosequencing were coupled with a detailed analysis of soil physicochemical properties to characterize archaeal diversity and identify environmental factors that might shape and maintain archaeal communities in soils of the three southern most McMurdo Dry Valleys (Garwood, Marshall, and Miers Valley). Archaea were successfully detected in all inland and coastal mineral soils tested, revealing a low overall richness (mean of six operational taxonomic units [OTUs] per sample site). However, OTU richness was higher in some soils and this higher richness was positively correlated with soil water content, indicating water as a main driver of archaeal community richness. In total, 18 archaeal OTUs were detected, predominately Thaumarchaeota affiliated with Marine Group 1.1b (> 80% of all archaeal sequences recovered). Less abundant OTUs (2% of all archaeal sequences) were loosely related to members of the phylum Euryarchaeota. This is the first comprehensive study showing a widespread presence and distribution of Archaea in inland Antarctic soils. Copyright 2014 Federation of European Microbiological Societies.AcknowledgementsnzTABS was supported by funding from the Foundation for Research, Science and Technology and the International Centre for Terrestrial Antarctic Research. We acknowledge Antarctica New Zealand for logistics and their overriding support for the project. We especially thank Eric Bottos and Sarah Kelly (Waikato University) for assistance with DNA extraction and sample preparation for 454 pyrosequencing that was carried out at the Waikato DNA Sequencing Facility, Hamilton, New Zealand. We thank Steve Pointing for help with the manuscript. This research was also partially supported by a National Science Foundation grant to SCC and JEB (ANT-0229836). The New Zealand Marsden Fund provided financial support for CWH, IRM, and SCC (UOW0802) and CKL (UOW1003).

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1st AuthorRichter, I.AuthorRichter, I.Herbold, C.Lee, C.McDonald, I.Barrett, J.Cary, S.Year2014JournalFEMS Microbiology EcologyVolume89Number2Pages347-359DOI10.1111/1574-6941.12322URLhttps://www.scopus.com/inward/recor.....70e86a0c3366ca6cccdc30550KeywordsArchaeaEuryarchaeotaOtus, adaptationAntarctic soilsAntarcticaarchaeonarticlebiodiversitychemistrydesiccationDNA sequenceEuryarchaeotageneticsmicrobiologymolecular geneticsphylogenypyrosequencingrestriction fragment length polymorphismsoilThaumarchaeota, Antarctic soilsArchaeaEuryarchaeotapyrosequencingThaumarchaeota, Adaptation, BiologicalAntarctic RegionsBiodiversityDesiccationEuryarchaeotaMolecular Sequence DataPhylogenyPolymorphism, Restriction Fragment LengthSequence Analysis, DNASoilSoil Microbiology, rank5Author KeywordsAntarctic soilsArchaeaEuryarchaeotaPyrosequencingThaumarchaeota

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TypeArticleCitationRichter, I., Herbold, C., Lee, C., McDonald, I., Barrett, J. and Cary, S. (2014) Influence of soil properties on archaeal diversity and distribution in the {McMurdo Dry Valleys, Antarctica}. FEMS Microbiology Ecology, 89(2): 347-359 doi:10.1111/1574-6941.12322 IdentifierRichter2014Relevancerank5

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Cary, S.