Chemical synthesis of a masked analogue of the fish antifree
Details of Research
TitleChemical synthesis of a masked analogue of the fish antifreeze potentiating protein (AFPP)AbstractA recently identified Antarctic fish protein termed antifreeze potentiating protein (AFPP) is thought to act as an adjunct to the previously characterised antifreeze glycoproteins (AFGPs), the two acting together to inhibit ice crystal growth in vivo. Elucidating the functional properties of the new AFPP requires access to large amounts of pure product, but the paucity of natural material necessitates alternative approaches. We therefore embarked on the total chemical synthesis of the AFPP, through a convergent ligation strategy. After many challenges, mostly due to the solubility issues of the peptide fragments, and several revisions of the original synthetic strategy, we have successfully synthesized a masked analogue of AFPP. The key to the successful synthesis was the use of a solubilising tag attached through a hydrolysable linker. â"' 2013 The Royal Society of Chemistry.AcknowledgementsThis research was supported by the Human Frontier Science Programme (grant RGP003/2009-C).
TypeArticleCitationYang, S.-H., Wojnar, J., Harris, P., Devries, A., Evans, C. and Brimble, M. (2013) Chemical synthesis of a masked analogue of the fish antifreeze potentiating protein (AFPP). Organic and Biomolecular Chemistry, 11(30): 4935-4942 doi:10.1039/c3ob41066h IdentifierYang2013Relevancerank3
Brimble, M., Chemical synthesis of a masked analogue of the fish antifree, [Yang2013]. Antarctica NZ, accessed 26/03/2025, https://adam.antarcticanz.govt.nz/nodes/view/63831, 10.1039/c3ob41066h
