Taxonomic and functional diversity of soil and hypolithic mi

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TitleTaxonomic and functional diversity of soil and hypolithic microbial communities in Miers Valley, McMurdo Dry Valleys, AntarcticaAbstractThe McMurdo Dry Valleys of Antarctica are an extreme polar desert. Mineral soils support subsurface microbial communities and translucent rocks support development of hypolithic communities on ventral surfaces in soil contact. Despite significant research attention, relatively little is known about taxonomic and functional diversity or their inter-relationships. Here we report a combined diversity and functional interrogation for soil and hypoliths of the Miers Valley in the McMurdo Dry Valleys of Antarctica. The study employed 16S rRNA fingerprinting and high throughput sequencing combined with the GeoChip functional microarray. The soil community was revealed as a highly diverse reservoir of bacterial diversity dominated by actinobacteria. Hypolithic communities were less diverse and dominated by cyanobacteria. Major differences in putative functionality were that soil communities displayed greater diversity in stress tolerance and recalcitrant substrate utilization pathways, whilst hypolithic communities supported greater diversity of nutrient limitation adaptation pathways. A relatively high level of functional redundancy in both soil and hypoliths may indicate adaptation of these communities to fluctuating environmental conditions. Copyright 2016 Wei, Lacap-Bugler, Lau, Caruso, Rao, de los Rios, Archer, Chiu, Higgins, Van Nostrand, Zhou, Hopkins and Pointing.

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1st AuthorWei, S. AuthorWei, S.Lacap-Bugler, D.Lau, M.Caruso, T.Rao, S.de los Rios, A.Archer, S.Chiu, J.Higgins, C.Van Nostrand, J.Zhou, J.Hopkins, D.Pointing, S.Year2016JournalFrontiers in MicrobiologyVolume7NumberOCTDOI10.3389/fmicb.2016.01642URLhttps://www.scopus.com/inward/recor.....e19ae2c9667678271307038abPublisherFrontiers Media S.A.KeywordsRNA 16S, ActinobacteriaAntarcticaarchaeonArticlebacterial metabolismcarbon fixationcontrolled studycyanobacteriumDNA extractionhigh throughput sequencinghypolithmicroarray analysismicrobial communitymicroclimatenitrogen transformationnonhumanphylogenypolymerase chain reactionpyrosequencingrestriction fragment length polymorphismsoil analysisstresssubstrate utilization, rank5Author KeywordsAntarcticaCyanobacteriaDry ValleysGeochipHypolithSoil

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CitationWei, S., Lacap-Bugler, D., Lau, M., Caruso, T., Rao, S., de los Rios, A., Archer, S., Chiu, J., Higgins, C., Van Nostrand, J., Zhou, J., Hopkins, D. and Pointing, S. (2016). Taxonomic and functional diversity of soil and hypolithic microbial communities in Miers Valley, McMurdo Dry Valleys, Antarctica. Frontiers in Microbiology, 7(OCT)Relevancerank5